Detailed information on the 929 biological respiration and oxygen consumption analysis system

Six channel aquatic organism respiration and oxygen consumption analysis system 929 model

The 929 model is a system that can repeatedly test dissolved oxygen, store data, and analyze it through a PC. Applying this system can save time for respiratory experiments and dissolved oxygen monitoring. A set of 929 system includes: 1 929 host, 1-6 1302 oxygen electrodes, electrode service accessories, and reactors (1 RC650 or 1-6 RC300, RC350, RC400 or other customized reactor containers). This system includes a microcomputer interface that can receive 6 1302 oxygen electrodes and software for operation on a PC. This system has extremely high accuracy, and different analytical reactors provide different uses. All technologies are in a global position, and products are distributed all over the world.

■ Features:

• 6 electrodes can be configured for convenient parallel experiments
• All oxygen pressure or concentration units can be selected
• Can perform all operations, including calibration on the monitor
• Display of recording ceremony style
• Facilitating the insertion of markers during the recording process
• High precision analysis, calculating standard respiratory rates
• Can be used in conjunction with a closed reactor or flow through a reactor
• Can conduct OECD209 standard experiments

Advantages:

• High precision research instruments
• Multiple different reactors
• Single channel, multi-channel
• The world's first respiratory apparatus applied to mitochondria
• Highly sensitive micro cathode oxygen electrode
• Simple and easy-to-use software

■ Performance:

• 1302 electrode repeatability: 0.1% (in water saturated with air)
• Polarization voltage: 400-900mV (anode grounded)
• Voltage: 100—250V， AC， 50 or 60Hz
• Size: 275×258×117mm

1302 micro cathode oxygen electrode

■ Features:

• Can be used in mixed and non mixed media
• Extremely high stable output
• Extremely low oxygen consumption rate
• Compatible with all respirator accessories

This is a Clark polarographic electrode with a 22 micron diameter platinum cathode and silver chloride anode connected by a buffered sodium chloride electrolyte.

In a typical structure, the cathode is shielded with a low permeability polypropylene membrane in order to allow the electrode to be used in solutions without stirring or solutions that require minimal stirring. With these membranes, the response time is correspondingly slower. To obtain a rapid response, it is necessary to prepare a fast respiratory enzyme preparation, using a very thin FEP (fluorinated ethylene propylene) membrane (with a special electrode sheath P/N SI 035), and the stirring of the medium must also be relatively fast. These electrodes do not have temperature compensation and must be used within a controlled temperature range (within ± 0.05 ℃). They are applied in an electrode sheath of the StrathKelvin attachment, so only the top of the electrode is exposed to the medium.

■ Performance:

Reaction time at 37 ℃

• Polypropylene film: 18 seconds (with a 90% change)
• FEP film: 6 seconds (for 90% change)
• Oxygen consumption (polypropylene film): 0.5—3×10-10mg O2/min
• Temperature coefficient: 2％/℃

Optional respiratory reactors of various types

RC400 respiratory reactor

Application: Testing respiration rate of large aquatic animals, including mussels and fish fry.

characteristic:

• The reactor capacity is 730ml.
• The design of the reactor ensures minimal interference from animals during the experimental process.
• The minimum stirring required to measure respiratory rate. If the animal is moving, there is no need to stir it.

RC300 and RC350 respiratory reactors

Application: Can be used for small-scale testing of OUR (oxygen consumption rate) in microbial suspensions, cell suspensions, mitochondria, or enzymes.

characteristic:

• Small sample capacity.
• Transparent reactor.

Performance:

Performance RC300 RC350

Reactor diameter: 13mm 16mm

Reactor volume: 0.3-1.0mL 1.0-3.0mL

Reactor: Glass Glass

Electrode sheath: Black ethylene glycol Black ethylene glycol

RC650 respiratory reactor

Application: Parallel measurement of respiratory reactions such as cell suspensions, microbial suspensions, mitochondria, or enzyme preparations. The breathing apparatus includes a sealed water bath that contains six reactors placed on a magnetic stirrer. A black formaldehyde electrode sheath with a 5mm small hole allows the top of the 1302 electrode membrane to pass through. Using the provided excellent accessory drilling needle, respiratory suppressants or other solutions can be directly injected into the reactor, and the volume of the reactor can be set between 1-3ml.

characteristic:

• Parallel experiments can be conducted using the advanced storage and analysis capabilities of the StrathKelvin 929 system.
• Complete unit, including electromagnetic stirrer.
• Visibility of containers.
• Variable reactor volume, 1-3 ml.

Performance:

• Unit diameter: 16mm
• Unit volume: 1-3 ml (adjustable)
• Unit: Glass with precise small holes
• Electrode: Black Acetal
• Water bath: Clear black acrylic acid
• Electromagnetic mixer: 6-position; 12VDC power supply from the top of the plug; FIXED SPEED

■ Attachment: 929 System Supporting Device Diagram